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KMID : 1037120220400020316
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The World Journal of Men′s Health
2022 Volume.40 No. 2 p.316 ~ p.329
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CD4+/CD8+ Ratio and Growth Differentiation Factor 8 Levels in Peripheral Blood of Large Canine Males Are Useful Parameters to Build an Age Prediction Model
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Lee Han-Jun
Hong Seok-Jin
Kim Seung-Soo
Kwon Young-Yon
Choi Bong-Hwan
Choi Kyung-Mi
Sheen Seo-Hyeong
Lee Myung-Jin
Hwang Sun-Young
Park Kyoung-Wan
Joo Young-Hun
Song Hwa-Young
Lee Cheol-Koo
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Abstract
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Purpose: To build an age prediction model, we measured CD4+ and CD8+ cells, and humoral components in canine peripheral blood.
Materials and Methods: Large Belgian Malinois (BGM) and German Shepherd Dog (GSD) breeds (n=27), aged from 1 to 12 years, were used for this study. Peripheral bloods were obtained by venepuncture, then plasma and peripheral blood mononuclear cells (PBMCs) were separated immediately. Six myokines, including interleukin (IL)-6, IL-8, IL-15, leukemia inhibitory factor (LIF), growth differentiation factor 8 (GDF8), and GDF11 were measured from plasma and CD4+/CD8+ T-lymphocytes ratio were measured from PBMC. These parameters were then tested with age prediction models to find the best fit model.
Results: We found that the T-lymphocyte ratio (CD4+/CD8+) was significantly correlated with age (r=0.46, p=0.016). Among the six myokines, only GDF8 showed a significant correlation with age (r=0.52, p=0.005). Interestingly, these two markers showed better correlations in male dogs than females, and BGM breed than GSD. Using these two age biomarkers, we could obtain the best fit in a quadratic linear mixed model (r=0.77, p=3×10-6).
Conclusions: Age prediction is a challenging task because of complication with biological age. Our quadratic linear mixed model using CD4+/CD8+ ratio and GDF8 level showed a meaningful age prediction.
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KEYWORD
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Age prediction model, Aging, CD4-CD8 ratio, Dogs, Myostatin
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